Composite

Part:BBa_K3467013:Design

Designed by: Wang Ran   Group: iGEM20_CAU_China   (2020-10-25)


T7+CHS dsRNA sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 611
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 611
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 671
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 611
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 611
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a dsRNA composite part and we only created a forward part. If future team wants to use dsRNA, team should add T7 promoter downstream and use RNAi express kit.


Source

doi: 10.3864/j.issn.0578-1752.2012.05.007

References